Probably the most common and annoying problem encountered in a cell culture laboratory is biological contamination. Contamination can be a minor nuisance or a major catastrophe depending on the type of contamination present and the culture method and volume utilized. Today reputable media and sera providers do a very good job of filtering their products using specialized filtration systems and therefore most if not all contamination is due to problems with technique, incomplete sterilization of equipment or some other failure. Prevention from biological contamination cannot be absolute, biological contamination does sometimes happen especially given that it is recommended that culturists do not use antibiotics.
It is possible to reduce the severity and frequency of biological contamination by following procedures such aseptic technique. What are your questions and comments about biological contamination and how can we help you overcome contamination problems in your lab.