Cytiva™ Protein Select™ Resin – A new affinity resin for recombinant proteins

Cytiva™ Protein Select™ resin is an affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva™ Protein Select™ tag. This technology simplifies tagged protein purification when used in research and standardizes purification during process development and later phases of any protein that does not have an affinity binding partner.

Key features and benefits:

  • Simple affinity purification protocol for recombinant proteins.
  • One-step purification and tag cleavage without the need for proteases or other elution reagents.
  • Delivers a highly pure, native protein with no remaining tag amino acids after tag cleavage.
  • Can be processed under mild conditions, which means no exposure to conditions that cause protein aggregation or loss of biological activity.
  • Flexible, enabling the use in multiple expression systems.

Simple Purification Protocol

Purifying a protein tagged with Cytiva™ Protein Select™ tag is simple. One chromatography step does the affinity purification, a traceless tag cleavage, and the tag removal. The pure protein obtained is in its native state with no traces of the tag amino acids. The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.

Recombinant Proteins: A family of very diverse molecules

The family of recombinant proteins includes very different types of molecules, such as enzymes, protein scaffolds, hormones, growth factors, etc. They often don’t have an affinity binding partner to facilitate their purification. To be able to standardize and simplify the purification with an affinity step, one strategy has been to add a tag to the recombinant protein.

Historically, using a tag had mainly been used for research purposes and not for biotherapeutic purification because traditional tags such as His-tag or GST-Tag could not be removed without leaving traces on the target molecules.

With Cytiva™ Protein Select™ resin and its corresponding tag, you can now use a tag purification protocol with confidence that the protein will be automatically removed from the tag and eluted with no residual tag amino acids.

Platform Process with Traceless Tag Affinity Purification to Accelerate Process Development

Adding Cytiva™ Protein Select™ tag to the recombinant protein enables the use of an affinity step with Cytiva™ Protein Select™ resin thereby standardizing and simplifying protein purification to obtain sufficient purity (see Fig 1). Process development gets easier, faster, more efficient. Fewer chromatography steps are required, which improves process time and cost.

Fig 1. Comparing processes using non affinity method in first step (A) vs a method using Cytiva™ Protein Select™resin (B) in first step. AIEX = anion exchange chromatography, CIEX = cation exchange chromatography, HIC = hydrophobic interaction chromatography

By utilizing the Cytiva™ Protein Select™ technology you can ensure that you will be reducing your process development time with no residual tag amino acids left behind. To learn more about this game changing technology, please click below.

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