Ask The Expert - Is it possible the elimination of contaminated culture from bacterial, yeast, fungal etc.?
Published: September 11, 2013
Session Expert: Timothy Fawcett, BioTechnical Institute of Maryland
Yes, but usually it is difficult and hard on the animal cells. It also depends on the type of contamination. Of course the best solution is to have non-contaminated cells that are frozen away for just that reason. If you are talking about a primary culture, I suggest isolating the cells with a double antibiotic-antimycotic for the first part of the isolation then 1X for the rest. Without knowing more about your culture contamination I would suggest 1 X Pen-strep and fungizone (amphotericin B); (10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Fungizone is the 100 X concentration). The antibiotics penicillin and streptomycin prevent bacterial contamination due to their effective combined action against gram-positive and gram-negative bacteria. Fungizone prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast. It will take multiple passages to eliminate the contamination and normally I would suggest going back and forth between with the antibiotic/antimycotic cocktail and without to see if any contamination continues to grow. In the event that the contamination is not killed over a curing course then switching to another antibiotic and antimycotic will help.