Is it possible the elimination of contaminated culture from bacterial, yeast, fungal etc.?
This question is part of the following Ask The Expert session:
Methods for Reducing Biological Contamination Risks in Your Lab
Company: BioTechnical Institute of Maryland
Job Title: Director
Yes, but usually it is difficult and hard on the animal cells. It also depends on the type of contamination. Of course the best solution is to have non-contaminated cells that are frozen away for just that reason. If you are talking about a primary culture, I suggest isolating the cells with a double antibiotic-antimycotic for the first part of the isolation then 1X for the rest. Without knowing more about your culture contamination I would suggest 1 X Pen-strep and fungizone (amphotericin B); (10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Fungizone is the 100 X concentration). The antibiotics penicillin and streptomycin prevent bacterial contamination due to their effective combined action against gram-positive and gram-negative bacteria. Fungizone prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast. It will take multiple passages to eliminate the contamination and normally I would suggest going back and forth between with the antibiotic/antimycotic cocktail and without to see if any contamination continues to grow. In the event that the contamination is not killed over a curing course then switching to another antibiotic and antimycotic will help.