We use BAPNA to test for residual trypsin activity. How does your kit compare?
This question is part of the following Ask The Expert session:
Verifying Removal of Residual Production Enzymes from Your Final Product
Answered by:
Answer
The Residual Protein Trypsin kit is an antibody-based immunoassay which provides data indicating the quantity of Trypsin protein present in a sample. The BAPNA test detects the rate of enzyme activity in the sample, i.e. enzymatic cleavage of the BAPNA molecule. In other words, the BAPNA test indicates how much enzyme activity is present, whereas our Roche kit will tell you how much Trypsin protein is present (whether active or not). This is important as these inactive pieces may still be immunogenic and have potential consequences for patients and end product performance.
This post is sponsored by Roche Custom Biotech.