The harvest and clarification of cell culture bioprocess fluids removes cells and cell debris and provides clarified cell culture harvest fluid for downstream purification. The success of the clarification step can greatly impact efficiency and ease of downstream purification.
During this Ask the Expert session, we discussed strategies for verifying the removal of residual enzymes used in biomanufacturing, like trypsin and collagenase from the final product. Specific questions included questions regarding sample prep, timing of testing, benefits of using a kit, sensitivity and clinical grade material.
Dr. Jan Bruder talks about his work at the Max Planck Institute for Molecular Biology in Münster Germany and his group’s use of automated high throughput drug screening program for ALS and Parkinson’s Disease.
This week, Dr. Roger Noble, Ph.D., Lead Technical Support Consultant at Roche Custom Biotech, will be answering your questions regarding strategies for verifying removal of residual enzymes from your final product, including how to use the ELISA-based Roche Residual Protein Trypsin and Liberase kits.
Roche Custom Biotech launched two new products, the Residual Protein Liberase Kit and the Residual Protein Trypsin Kit, to address some of the key challenges associated with current residual enzyme testing methods.