Impact of Continuous Chromatography Mode on Protein A Resin Lifetime
The presented data demonstrates that PCC does not compromise the performance of MabSelect SuRe LX or MabSelect SuRe pcc over the performed 175 cycles, supporting the use of this technique to improve resin capacity utilization against batch processing. While the performance indicators (HCP, leached Protein A, and mAb content in eluate) were comparable between the resins, MabSelect SuRe pcc demonstrated an approximately 25% higher mAb binding capacity compared with MabSelect SuRe LX under the conditions used. The results confirm that the use of a resin designed for PCC can help improve economy of continuous processes even further.