Month: September 2020

The combination of using a low pressure ion exchange step (50-100 micron) with standard reverse phase (12-18 micron) polishing was demonstrated using Chromalite® PCG1200FS and Chromalite® 15AD2. This resulted in a peptide purification process that was more cost-effective than the traditional two-step reverse phase process. The is because the low pressure ion exchange step is much more cost effective than the reverse phase step. This novel process permitted bivalirudin purification from a semi-crude feed of 80% purity to greater than 99% purity with greater than 90% recovery.

Technologies like the Praesto® Jetted A50 resin and novel approaches for the chromatography step provide the flexibility needed to meet the increasing demands placed on downstream processing. These novel approaches have been shown to provide increased productivity, decreased buffer consumption and lower cost for consumables. These strategies and others will be needed to meet the evolving demands for mAb manufacturing and enable more efficient use of both existing and future downstream facilities.